pathwayTools版本过老学术讨论回答质疑

"Thank you for the comment. In our study, metabolic pathway annotation was conducted using three independent tools (KEGG, Reactome, and PathwayTools) to ensure robustness. While PathwayTools 24.0 was used, the core metabolic pathways were cross-validated with KEGG (latest release) and Reactome databases. The consistency across multiple annotation tools supports the reliability of our findings. Key pathways identified were further validated by [GO/KEGG enrichment analysis / RNA-seq expression data / experimental validation]."